Negative acts could be considered indexes of mobbing risk in workplace. Researching on these events is useful to plan interventions to prevent mobbing cases. In this research (N = 164), main aims are to study negative acts in an organization and to investigate whether these events are associated with work variables (i.e. roles, departments) and perceptions of organizational and subjective well-being. Results show that negative acts pertaining to Work related bullying were more frequent than negative events pertaining to Personal Bullying. Moreover different level of negative acts were found in different departments of the organization. Generally, in the departments where there were higher levels of Negative acts we found lower level of organizational and subjective well-being. Keywords: negative acts, mobbing, work, mobbing risk, organizational well-being.